Coding

Part:BBa_K1486007:Design

Designed by: iGem EPFL 2014   Group: iGEM14_EPF_Lausanne   (2014-08-25)


IFP[2]

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 305
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 305
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 516
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 305
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 305
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal PstI site found at 305


Design Notes

When fused to another coding sequence, insert a flexible linker (check out our linker BBa_K1486003 for example).

Source

We received Michnick lab IFP1 and IFP2 sequences from addgene.

References

Michnick, S., Tchekanda, E., & Sivanesan, D. (2014, April 20). An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nature Methods, 6-6.