RBS (Arabidopsis Thaliana).
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
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- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
We had to discuss what was the best option to enhance the expression efficiency of our proteins of interest to bio-remediate heavy metals, as mercury. As we already know, in eukaryotes the ribosome binding site (RBS) promotes efficient and accurate translation of mRNA. Studies have shown that every eukaryotic specie has its nucleotide preferences, wich means that each specie has its own Kozak sequence. We designed this BioBrick to perform the best protein expression possible in Arabidopsis thaliana.
Arabidopsis thaliana genome sequence.
Rangan, et al. (2008). Analysis of Context Sequence Surrounding Translation Initiation Site from Complete Genome of Model Plants. New York University. [Online] Retrieved october 14th 2014 from: http://www.nyu.edu/projects/vogel/Reprints/Rangan_MolBt08.pdf
Nakagawa, et al. (2007). Diversity of preferred nucleotide sequences around the translation initiation codon in eukaryote genomes. Oxford University Press. [Online] Retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2241899/
Liu Q, Xue Q. (2005). Comparative studies on sequence characteristics around translation initiation codon in four eukaryotes. Zhejiang University. [Online] Retrieved october 14th 2014 from: http://www.ias.ac.in/jgenet/Vol84No3/317.pdf
Kozak, M. (1989). Circumstances and mechanisms of inhibition of translation by secondary structure in eucaryotic mRNAs. American Society for Microbiology (ASM). [Online] retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC363665/