RBS

Part:BBa_K1471011:Design

Designed by: Juan Noe Hernandez Salazar   Group: iGEM14_Biosint_MEXICO   (2014-10-14)


RBS (Arabidopsis Thaliana).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to discuss what was the best option to enhance the expression efficiency of our proteins of interest to bio-remediate heavy metals, as mercury. As we already know, in eukaryotes the ribosome binding site (RBS) promotes efficient and accurate translation of mRNA. Studies have shown that every eukaryotic specie has its nucleotide preferences, wich means that each specie has its own Kozak sequence. We designed this BioBrick to perform the best protein expression possible in Arabidopsis thaliana.

Source

Arabidopsis thaliana genome sequence.

References

Rangan, et al. (2008). Analysis of Context Sequence Surrounding Translation Initiation Site from Complete Genome of Model Plants. New York University. [Online] Retrieved october 14th 2014 from: http://www.nyu.edu/projects/vogel/Reprints/Rangan_MolBt08.pdf

Nakagawa, et al. (2007). Diversity of preferred nucleotide sequences around the translation initiation codon in eukaryote genomes. Oxford University Press. [Online] Retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2241899/

Liu Q, Xue Q. (2005). Comparative studies on sequence characteristics around translation initiation codon in four eukaryotes. Zhejiang University. [Online] Retrieved october 14th 2014 from: http://www.ias.ac.in/jgenet/Vol84No3/317.pdf

Kozak, M. (1989). Circumstances and mechanisms of inhibition of translation by secondary structure in eucaryotic mRNAs. American Society for Microbiology (ASM). [Online] retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC363665/