MotA and B0032 RBS
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 342
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 80
- 1000Illegal SapI.rc site found at 848
To make the RBS + motA biobrick, motA was amplified by PCR using the proofreading Phusion polymerase using DS941 genomic DNA as template. The forward primer incorporated the prefix, added the BBa_B0032 ribosome binding site (RBS) and a scar sequence just upstream of motA and changed the natural GTG start codon to ATG. The reverse primer incorporated the suffix and changed the stop codon to TAA.