Switch Nucleotide Spacer
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Created this part with 2 restriction sites when combined with scar sites in larger composite part.
Restriction sites in switch - HindIII
HindIII was designed into the switch downstream of the promoter. This allowed for confirmation of the "flip" of the switch by restriction digest.
Using both HindIII and PstI produces different sized fragments dependent on the orientation of the switch promoter.
Synthesised by IDT Technologies from a given sequence. Sequence is not from genome.