Anderson Promoter + nixA + Ter + pT7 + GST-CRS5 + Ter
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21Illegal BamHI site found at 1801
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal SapI.rc site found at 1213
Expression of GST-CRS5 is controlled by the bacteriophage T7 promoter, so part should be combined with a host organism expressing T7 polymerase (such as E. coli BL21-AI from Life Technologies).
This part is a composite of BBa_K1460003 and BBa_K1460001.