Anderson Promoter + RBS + CBP4
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21Illegal BglII site found at 684
Illegal BamHI site found at 279
Illegal BamHI site found at 371
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
The Anderson promoter will provide strong constitutive expression of the CBP4 gene in E. coli.
Anderson promoter is that of part BBa_J23102. CBP4 is a naturally found coding sequence in Nicotiana tabacum. This part was synthesized by BioBasic and was transferred into pSB1C3 using standard cloning procedures.
 Arazi, T., Sunkar, R., Kaplan, B., & Fromm, H. (1999). A tobacco plasma membrane calmodulin-binding transporter confers Ni2 tolerance and Pb2 hypersensitivity in transgenic plants. The Plant Journal, 171-182