Designed by: Eric Holmes Group: iGEM14_Cornell (2014-08-14)
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Applications of BBa_K1460003
Cornell 2014 Results
Cells successfully expressing NixA should be transporting more nickel ions past the cell wall. This would lead to increased nickel sensitivity. To test for nickel sensitivity, E.coli BL21 and engineered BL21 with part BBa_K1460003 in the cmr plasmid pSB1C3 were grown for a 24 hour period in LB with 1 mM Ni.
What we observe after 24 hours of growth is no significant difference in growth between the two strains (top graph). What we consistently observed as well, however, is that there is no inhibition of growth of BL21 at high concentrations of nickel (bottom figure). Even if nixA is expressed and is actively transporting nickel ions into cells, it is possible that the concentration of nickel is still not high enough to be toxic to the organisms. We were, unfortunately, unable to test nickel concentrations higher than those shown above because these working concentrations are approaching the maximum solubility of the nickel (II) chloride that we were using for testing.