Coding

Part:BBa_K1446001

Designed by: Philipp Lorenz   Group: iGEM14_Oxford   (2014-09-29)


dcmA-gene with N-terminal pdu-microcompartment tag, codon optimised for E.coli (K12)

dcmA is a gene coding for the enzyme dichloromethane dehalogenase from Methylobacterium extorquens (DM4). This enzyme is a member of the glutathione-dependent-S-transferase family and catalyses the following reaction: DCM (dichloromethane) + 2H2O -> formaldehyde + 2 HCl We have attached the N-terminal targeting sequence for the pdu-microcomartment to this part in order to allow for the insertion of this enzyme into the microcompartment.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 238
    Illegal AgeI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None