Designed by: Caroline de Cock Group: iGEM14_Warwick (2014-10-07)
NKRF IRES with forward GFP
This was the complete testing module used in the testing of the NKRF IRES, results can be seen onPart:BBa_K1442024. This uses the empty testing GFP sequence Part:BBa_K1442106, which has been codon optimised for human use using the IDT codon optimiser from the original gene derived from Aequorea victoria jellyfish gene Part:BBa_E0040.
This was measured in Huh 7.5 and HeLa cell with a tecan magellan machine.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 1289
- 1000Illegal BsaI.rc site found at 375
Illegal SapI site found at 734