Regulatory

Part:BBa_K1437004

Designed by: Yifan Wu   Group: iGEM14_HIT-Harbin   (2014-09-30)

E-LexAop+CYC1 promoter

E-LexAop is 8 repeated sequences which has more power to combine the lexA DBD and let the CYC1 Promoter has more efficiency to induce the expression of the downstream sequences. It is designed by Professor Mario Andrew and because it has 8 binding domain with lexA DBD.Theorily it is 16 times faster then the lex Aop erator with 4 binding sequences. It is designed to work good in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None