5' Integration sequence for the amyE locus of B. subtilis
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
The amyE integration sequence was taken from the vector after comparison by BLAST to the B. subtilis chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 5' end of the gene is the 5' sequence required for integration
B. subtilis integration plasmid pDR111#1
5'- GCT CTA GAG ATG TTT GCA AAA CGA TTC AAA AC - 3'
5'- GGA CTA GTA TCG ATC AGA CCA GTT TTT AAT TTG -3'
The 5’ integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on B. subtilis, in particular in the studies of transcriptional control#2,#3,#4
- 1 pmid=12562810
- 2 pmid=14597697
- 3 pmid=15937167
- 4 pmid=12169614