σx Generator + PcomFA-E0240
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 359
Illegal BsaI.rc site found at 1550
- - PcomFA primers sequence was obtained from Wellcome Trust Sanger Institute, a British genomics and genetics research institute.
- iGEM 2014 Hong_Kong_HKUST Team has cloned PomFA from S. pneumoniae strain NCTC7465 by PCR. Then, restriction-ligation with σX generator BBa_K1379006 and BBa_E0240 in pSB1C3 backbone was performed.