Composite

Part:BBa_K137056

Designed by: Victoria Hsiao   Group: iGEM08_Caltech   (2008-07-31)


folB + folKE + b0015 in psb2k3

This is the complete construct of two of the five folate biosynthesis genes from L.lactis. We hope to use this plasmid to test the effects of overexpression of the folate gene cluster on total folate production in E. coli.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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