Composite

Part:BBa_K137055

Designed by: Victoria Hsiao   Group: iGEM08_Caltech   (2008-07-28)

Constitutive pabA expression construct

pabA is one of three genes (pabA, pabB, pabC) involved in the para-aminobenzoic acid (pABA) synthesis pathway from chorismate. In this construct, pabA (para-aminobenzoate synthetase component II) has been placed behind the strong promoter J23100 and the strong RBS B0034, and in front of the terminator B0015. Currently it is in the high copy plasmid pSB1AK3.


Usage and Biology

When measured using the Caltech iGEM PABA HPLC protocol, K137055 in pSB1AK3 in DH10B produced 700ng/mL of PABA (vs. negligible production for DH10B without K137055).

PABA.png

HPLC trace. PABA elutes at 4.8 minutes. Blue: DH10B (negative control), Green: K137055

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None