Coding

Part:BBa_K1364019:Design

Designed by: Mathieu Fourniť   Group: iGEM14_Toulouse   (2014-10-08)


Pveg + RBS + Antifungal EcAMP + Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is composed of the constitutive promoter Pveg and strong RBS for B. subtilis (http://parts.igem.org/Part:BBa_K733013"), the revised open reading frame of EcAMP-1 (with a stop codon) and a double terminater (http://parts.igem.org/Part:BBa_B0015).
The EcAMP-1 part was codon optimized for E. coli by the iGEM Utah State team and thanks to the Life Technologies GeneArt software program.

BBa_K1364011.png

Source

The iGEM Utah State team in 2013

References

Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli). The journal of Biological Chemistry. 2011, Vol. 286, 25145‚Äď25153