N-acetylatedGlucosamine based chemotaxis for Bacillus subtilis
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1913
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 1370
Illegal SapI.rc site found at 2006
The chimeric protein contains the intracellular domains of McpA from Bacillus subtilis to enable the transduction of the signal: these domains correspond to the amino acids 1-37 and 282-610. The extracellular domain of VCD_000306 is inserted between the intracellular regions of McpA to sense N-acetylglucosamine and corresponds to the amino acids 31-310. Its expression is optimized for in Bacillus subtilis. This part is also composed of a Strong RBS (K780002) and a terminator (B0015) . The codon optimization was made thanks to the DNA 2.0 software program.
This gene was ordered from a synthesis company.
K. Meibom,L. Xibing, A. Nielsen, CY. Wu, S. Roseman and G. Schoolnik. The Vibrio cholerae chitin utilization program . The National Academy of Sciences of the USA (2004).
C. Kristich and GW. Ordal.Bacillus subtilis CheD is a chemoreceptor modification enzyme required for chemotaxis. J Biol Chem. 2002 Jul 12;277(28):25356-62. Epub 2002 May 13.