Composite
1GateNpuE

Part:BBa_K1362101:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer   Group: iGEM14_Heidelberg   (2014-10-08)


RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 958
    Illegal AgeI site found at 1070
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1247
    Illegal BsaI.rc site found at 172


Design Notes

???


Source

The part is assembled from different sources. The intein sequences come addgene or were kindly provided by different working groups. BBa_J04450 was used as mRFP selection marker. The scars and overhangs are part of the RFC[105] intein cloning strategy and are further explained on our wiki and in RFC[105] [1]. Please see the corresponding basic parts for the individual detailed information.

References