RBS + NpuDnaE N-intein RFC assembly construct (with His6)
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 826
Illegal AgeI site found at 938
- 1000Illegal BsaI site found at 1115
Illegal BsaI.rc site found at 40
The part is designed in compliance with RFC . For an exact description of how to use it or how to design other intein constructs accordingly, please read the iGEM team Heidelberg 2014's wikipage or the RFC[???].
The part is assembled from different sources. The intein sequences come addgene or were kindly provided by different working groups. BBa_J04450 was used as mRFP selection marker. The scars and overhangs are part of the RFC intein cloning strategy and are further explained on our wiki and in RFC . Please see the corresponding basic parts for the individual detailed information.
 [link RFC]
 Zettler, J., Schütz, V. & Mootz, H. D. The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction. FEBS Lett. 583, 909–14 (2009). http://www.ncbi.nlm.nih.gov/pubmed/19302791