Composite
Part:BBa_K136014
Designed by: alexandra bouaziz Group: iGEM08_Paris (2008-10-25)
AracpBAD - gfp triparts
pBad is an E. coli promoter that is tightly controlled by:
*inducer: L-arabinose.
* repressor: AraC apparently acts as the repressor
one study concluded that arabinose can change the conformation of araC and prevent it from successfully binding to and repressing pBad. pBad is followed by a GFP tripart in our construction .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2091
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2030
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1865
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 665
Illegal SapI site found at 1847
[edit]
Categories
Parameters
None |