Part:BBa_K136010

fliA promoter

FliA is a transcription factor involved in the formation of the flagella in E. coli. The promotor of fliA is induced by FlhDC. FliA seems to have also a positive autoregulation on its promotor.
We have introduced mutations in the sequence of fliA promotor in order to get rid of the PstI and EcoRI restriction sites.

WARNING
The promoter cloned here corresponds to the promoter region given in the following paper:
Kalir, S. et al. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science 292, 2080-2083 (2001)

Position 2000123-1999779 in MG1655 genome sequence.

Note that the promoter region given here includes the beginning of the fliA protein. It thus cannot be used with any protein coding biobrick since it will add a small peptide and most probably produce a frame shift in your protein. To use it in front of an ORF, this ORF must be preceded by a stop codon in the right frame.

We are currently building a new version of this promoter which will include stop codons so that it will comply with the biobrick standard and be usable in front of any protein coding biobrick. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]