Composite

Part:BBa_K1357001

Designed by: Matthew Tucker   Group: iGEM14_Virginia   (2014-10-06)

Manganese Peroxidase With pelB Secretion Tag

This part contains the coding sequence for the enzyme Mangaenese Peroxidase (MnP) as well as the secretion tag pelB. Manganese peroxidase is an enzyme from the fungus, Phanerochaete chrysosporium. In this fungus, commonly known as white rot fungus, this enzyme aids in lignin degradation. This enzyme has been shown to degrade nylon 6 and nylon 66. The sequence for MnP in this BioBrick has been codon optimized for E.coli. A pelB secretion tag was also attached to the MnP sequence. It is intended for use by teams that are exploring plastic degradation in their research. Enzymatic activity requires a pH near 4.5 as well as a manganese source such as manganese sulphate. Works best at temperatures near 32 C. Related genes include nylC, nylon hydrolaze, which is also used to degrade nylon plastics.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 972
    Illegal AgeI site found at 721
    Illegal AgeI site found at 766
    Illegal AgeI site found at 964
    Illegal AgeI site found at 994
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None