Regulatory

Part:BBa_K1323021

Designed by: Julia Manalil   Group: iGEM14_Waterloo   (2014-10-08)

sarA P1 Staphylococcus aureus Constitutive Promoter

This sarA P1 promoter is part of the sar locus from S.aureus strain RN6390 (Bayer et al., 1996). This locus contains three transcripts (sarA, sarB and sarC) and they are expressed under three separate promoters (Bayer et al., 1996). Moreover, each one of these promoters is capable of generate transcripts on their own (Bayer et al., 1996). This constitutive promoter has been shown to optimize expression of fluorescent marker such as YFP, GFP and mCherry (Malone et al., 2009, & Fey, 2014). The sequence of the sarA P1 promoter came from 1996 paper published by Bayer et al.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Bayer, M. G., Heinrichs, J. H., & Cheung, A. L. (1996). The molecular architecture of the sar locus in Staphylococcus aureus. Journal of Bacteriology, 178(15): 4563-70.

Fey, P. D. (2014). Staphylococcus epidermidis: methods and protocols. New York: Springer Science + Business Media, LLC.

Malone, C. L., Boles, B. R., Lauderdale, K. J., Thoendel, M., Kavanaugh, J. S., & Horswill, A. R. (2009). Fluorescent Reporters for Staphylococcus aureus. Journal of Microbiological Methods, 77(3): 251-260.


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