Composite

Part:BBa_K1322001

Designed by: Ophir Ospovat   Group: iGEM14_Vanderbilt   (2014-10-15)


Self-Regulating Oscillating Fluorescent System

See BBa_K546546 for function. This part was optimized through site-directed mutagenesis of specific restriction sites in order to make the original part RFC[21] and RFC[25] compatible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 2042
    Illegal BsaI.rc site found at 2930
    Illegal BsaI.rc site found at 3657


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Parameters
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