CBDcex fused to sfGFP with T7 promoter
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 53
- 1000Illegal SapI.rc site found at 401
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The T7 vector was fused to the protein fusion using the Xba and SpeI site.
CBDcex comes from Cellulomonas fimi, and sfGFP is a modified super-folding mutant of GFP. Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.
The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.