sfGFP fused to dCBD driven by LacI
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 230
- 1000Illegal SapI.rc site found at 245
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.
The LacI vector was fused to the protein fusion using the Xba and SpeI site.
The LacI part came from BBa_J04500.
Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.
dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.