Composite

Part:BBa_K1321357:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)


sfGFP fused to CBDcex driven by LacI

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The LacI vector was fused to the protein fusion using the Xba and SpeI site.

Source

The LacI part came from BBa_J04500.

Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.

CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.

References