Regulatory

Part:BBa_K1216009:Design

Designed by: Meret Arter   Group: iGEM13_ETH_Zurich   (2013-10-29)


Variant of the wild-type pLuxR promoter with lower sensitivity

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter was built through site-saturation mutagenesis of the wild-type promoter.


Source

LuxR binding site were mutated to reduce the sensitivity of the promoter.

References