Generator

Part:BBa_K1216002:Design

Designed by: Parvathi Chandran   Group: iGEM13_ETH_Zurich   (2013-08-29)

Acetyl esterase (aes) from Escherichia Coli

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NO design considerations.



We obtain the plasmid with the hydrolase from Johannes Haerle of the Panke Group from D-BSSE ETHZ in Basel, checked them for biobrick restriction sites and removed them. We sequenced them to confirm the sequence and did PCR with overlapping fragments to introduce the biobrick pre and suffix to clone them into biobrick vectors.

Source

Escherichia coli

References