Acetyl esterase (aes) from Escherichia Coli
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 27
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal SapI site found at 951
NO design considerations.
We obtain the plasmid with the hydrolase from Johannes Haerle of the Panke Group from D-BSSE ETHZ in Basel, checked them for biobrick restriction sites and removed them. We sequenced them to confirm the sequence and did PCR with overlapping fragments to introduce the biobrick pre and suffix to clone them into biobrick vectors.