Composite

Part:BBa_K117009

Designed by: Nguyen Xuan Hung   Group: iGEM08_NTU-Singapore   (2008-10-08)

colicin E7 production system induced by lactose

This system is induced by lactose to produce colicin E7 with Immunity protein.
For more insights on the functions of E7-Immunity gene, please visit our BBa_K117001 part

How does this system work?

In OFF state: pLacI is inhibited by its inhibitor LacI which is produced by the lac operon. As a result, E7-Imm gene cannot be expressed.
In ON state: upon adding of lactose, lacI is no longer able to bind to LacI promoter. Hence, lactose indirectly triggers the expression of E7-Imm.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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