Coding

Part:BBa_K117000:Design

Designed by: Nguyen Xuan Hung   Group: iGEM08_NTU-Singapore   (2008-10-07)

Lysis gene (promotes lysis in colicin-producing bacteria strain)

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lysis gene was commercially synthesized by Science Wereke

Extraction of Lysis gene/PCR Oligo fragments DNA sequence Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector.

Source of Orgin sequence

to be updated

Source

to be updated

References

Cloning and Characterization of ColE7 Plasmid. J Gen Microbiol 137:97-100

Characterization of the cea gene of the ColE7 Plasmid. Mol Gen Genet (1992) 233:177-183 "