- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1550
- 21Illegal BamHI site found at 1489
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 1324
- 1000Illegal SapI site found at 1306
HlyA C terminal part has a 5' section compatible with Silver Fusion standard. Downstream, HlyB and HlyD are both under the AraC/pBAD promoter to be induced by arabinose.
The HlyB and HlyD sequences come from the parts registry, while the HlyA from the registry had mayor problems, so we took it from the genomic sequence.