Part:BBa_K1159119

Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF3 version)

Red light triggered TEV Protease system with FRET Reporter for plants translation unit (PhyB/PIF3 version)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 487
  Illegal BamHI site found at 569
  Illegal BamHI site found at 3432
  Illegal BamHI site found at 4862
  Illegal XhoI site found at 520
  Illegal XhoI site found at 539
  Illegal XhoI site found at 2674
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 3849
  Illegal NgoMIV site found at 5279
  Illegal AgeI site found at 3196
  Illegal AgeI site found at 3551
  Illegal AgeI site found at 4626
  Illegal AgeI site found at 4981
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2059
  Illegal BsaI site found at 2465
  Illegal SapI site found at 736
  Illegal SapI.rc site found at 3151
  Illegal SapI.rc site found at 3629
  Illegal SapI.rc site found at 4170
  Illegal SapI.rc site found at 5059