PRM/lac is a hybrid promoter that is activated by lamda repressor (CI) and repressed by LacI repressor (Fig. 1).
On the downstream of the promoter, GFP is inserted as a reporter.
To characterize the function of the RM/lac hybrid promoter (BBa_K1139151), we constructed this part PRM/lac-GFP (BBa_K1139150) by inserting RM/lac promoter upstream of a GFP coding sequence. By using reporter cells that contain PRM/lac-GFP, we measured the fluorescence intensity of the cells induced by CI and IPTG (Fig. 2).
We saw that our new RM/lac hybrid promoter was actually activated by CI through an induction assay (Fig. 3).
For more information, see our work in Tokyo_Tech 2013 wiki.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 769