Coding

Part:BBa_K1122006:Design

Designed by: Lina Gasiūnaitė   Group: iGEM13_Edinburgh   (2013-08-13)


mCherry (BBa_J06504) compatible with Genabler assembly

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To be compatible with Genabler assembly, the part has inward-facing EarI sites at the ends. When assembled by BioBrick assembly, five N-terminal residues MetAlaSerSerSer- and one C-terminal Ala residue will be added to the original sequence of BBa_J06504. When assembled with normal BioBrick assembly, the part contains a start and a stop codon, however, if Genabler assembly is used, the resulting part does not contain either of the codons. Such design enables to use standardised linkers that contain start and stop codons and variable upstream sequence in Genabler assembly.

Source

mCherry is based on the BioBrick BBa_J06504.

References

Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY, Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004 Dec; 22(12):1567-72.