Composite

Part:BBa_K1106000

Designed by: Manuel Giménez   Group: iGEM13_Buenos_Aires   (2013-09-09)

mRFP generator under hybrid promoter: HSL-LuxR activated, P22 C2 repressed

Monomeric RFP: Red Fluorescent Protein under hybrid promoter activated by HSL-LuxR and repressed by P22 C2.

Usage and Biology

This construction permit to tune the expression of mRFP with the proper construction of LuxR and P22 c2.


This parts was originally built to be used in iGEM BsAs 2013 project to implement an arsenic sensor device based on synbio technology. Visit our wiki to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part. Igem.bsas.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 650
    Illegal AgeI site found at 762
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None