Regulatory

Part:BBa_K1104205:Experience

Designed by: Ting-Yun Chiang   Group: iGEM13_NYMU-Taipei   (2013-09-16)

This part: AhpCp2 is one of the improvement versions of BioBrick Part: ahpC promoter (Part:K362001) designed by 2010 KIT-Tokyo team. AhpCp2 (Part:BBa_K1104205) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:Sensing Nosema ceranae.

AhpCp2 +E0840 (Part:BBa_K1104245)

We use the device: AhpCp2 +E0840 (Part:BBa_K1104245) includes promoter (Part:BBa_K1104205) and Part:BBa_E0840, induce the promoter by different concentrations of H2O2, and identify the strength. After AhpCp2 (Part:BBa_K1104205) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.

We tested the improved promoter: AhpCp2 by following these Promoter Testing protocols. This wiki page shows the protocols we used for promoter testing.

Results

This is the relative promoter strength of BBa_K1104205 under different H202 concentrations tested using BBa_K11042045 (BBa_K1104205 +E0840) and found that there is a trend where the higher the H202 concentration, the higher the promoter expression:

NYMU AhpCp2 strength.png

Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:

Comparison between ahpC and its improvements. (Part:BBa_K1104245)

Promoter testing of ahpC promoter (Part:K362001) and its improvements.

NYMU AA.png
NYMU AAAA.png

Discussion

Applications of BBa_K1104205

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