Coding

Part:BBa_K106671:Design

Designed by: Jacinto Chen   Group: iGEM08_UCSF   (2008-10-28)


GFP, Aar1 AD part

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644
    Illegal SpeI site found at 1


Design Notes

As an AD part, there is no ATG, and the stop codon is included. For more information on AarI cloning, please refer to the UCSF 2008 igem page.


Source

PCR amplified from lab plasmid stocks.

References