lac_promoter + RBS + SAM synthetase
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 324
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 904
- 1000COMPATIBLE WITH RFC
The gene sequence was extracted using the following primers:
>Primer Forward: prefix + RBS + spacer + ATG...
>Primer Reverse: sequence_TAATAA_suffix
SAM synthetase was inserted into BBa_R0010 through standard assembly.
SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.
Ethylene biosynthesis and its regulation in higher plants
Yang, S. F.; Hoffman N. E. Ann. Rev. Plant Physiol. 1965, 35, 155-89.
- MetaCyc EC 18.104.22.168, MetaCyc. August 15th, 2013, from link
Ethylene biosynthesis and signaling networks
Wang, K. L.-C.; Li, H.; Ecker J. R. Plant Cell 2002, Supplement, S131-S151