Composite

Part:BBa_K1042015

Designed by: Parth Patel   Group: iGEM13_Gaston_Day_School   (2013-09-26)

Cadmium Responsive GFP+Ogr Activator

After creating two intermediate parts, the upstream one being the Cadmium Promoter with Ogr Activator (BBa_K1042012) and the downstream one being the PO Promoter with GFP (BBa_K1042013), we digested the intermediates and ligated them to the linearized pSB1C3 plasmid provided. The resulting colonies were tested for responsive GFP production from the addition of cadmium.

The addition of the Ogr Activator and PO Promoter act as a sensitivity tuner amplifying the Cadmium Promoter's signal and creating more GFP. This combination should make the part more sensitive.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 200
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1200


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