Coding

Part:BBa_K1039012:Design

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-12)


PhiC31 integrase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 51
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1356
    Illegal SapI.rc site found at 1414
    Illegal SapI.rc site found at 1519
    Illegal SapI site found at 1665


Design Notes

The following mutation was made: Base 51 is changed from T to C, silent mutation. The sequence was synthesized by a third party company.



Source

Synthesized DNA from BioBasic.

References