Protein_Domain

Part:BBa_K103003

Designed by: Michael Lower   Group: iGEM08_Warsaw   (2008-09-14)

B domain of Staphylococcal protein A

B domain of Staphylococcal protein A[http://www.ncbi.nlm.nih.gov/pubmed/7236608?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum], which specifically binds to the Fc portion of immunoglobulin G, it is also strong binder of ZSPA-1.


Structure of B domain of Staphylococcal protein A [http://www.rcsb.org/pdb/explore.do?structureId=1BDD]

Usage and Biology

  • Protein A is the famous protein from Staphylococcus aureus which binds to constant fragments of IgG antibodies and has many uses in molecular biology (TapTag for instance).
  • We have used this protein as hunter fused with BBa_K103017 or BBa_K103018 in our 'hunter and prey' selection system
  • This part uses our BamHI/SacI/NdeI fusion cloning substandard
 Protein a.jpg

source: [http://www.pdb.org/pdb/explore.do?structureId=1BDD PDB]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 91
    Illegal BamHI site found at 9
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/receptor
Parameters
None