Coding

Part:BBa_K1026001:Design

Designed by: Hongyi WU   Group: iGEM13_SJTU-BioX-Shanghai   (2013-09-16)


dCas9

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1340
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1340
    Illegal BamHI site found at 3378
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1340
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal EcoRI site found at 1340


Design Notes

Mutation: Aspartate 10 to Alanine (D10A) and Histidine 840 to Alanine (H840A) in order to cripple the endonuclease activity.


Source

The dCas9 comes from S. pyogenes.

References