Protein Phosphatase 1 with HA tag
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 527
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
The natural PstI site within the human PP1 gene was silently removed by site-directed mutagenesis
We thank Dr James Hastie (Division of Signal Transduction Therapy, College of Life Sciences, University of Dundee) for providing the pGEX6P-1 PP1 CAT gamma vector (Kelsall et al. 2011) encoding a GST fusion to PP1. This vector served as a PCR template to allow cloning of the PP1 coding sequence into pSB1C3, remove illegal restriction sites, and submit to the Registry under RFC standard.
Kelsall IR, Voss M, Munro S, Cuthbertson DJ, Cohen PT. (2011) R3F, a novel membrane associated glycogen targeting subunit of protein phosphatase 1 regulates glycogen synthase in astrocytoma cells in response to glucose and extracellular signals. Journal of Neurochemistry 118:596-610.