Intermediate

Part:BBa_K091199

Designed by: Xiao Zhu   Group: iGEM08_Davidson-Missouri_Western   (2008-08-15)


plasR/cI overall testing construct

using GFP to test the hybrid promoter plasR/cI with P-AI-1 receiver and lambda cI genes to repress the promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 2188
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2128
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1459
    Illegal AgeI site found at 1656
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 835


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Categories
Parameters
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