Generator

Part:BBa_J70304

Designed by: Tom Knight   Group: Knight Lab   (2009-05-06)

constitutive cat cassette for Mesoplasma florum

This part is a version of J70026, the cat cassette from pC194. The original cassette is inducible, with a 5' UTR containing a hairpin structure which sequesters the Shine-Dalgarno sequence. In this mutation, we eliminate the hairpin structure from the 5' UTR to attempt a constitutive construct.

This modified cassette is functional at 15 ug/ml chloramphenicol in Top10 E. coli cells. Slow growth and small colonies are evident on normal 35 ug/ml chloramphenicol plates.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/mflorum
Parameters
None