Coding

Part:BBa_J64004

Designed by: Dan Widmaier   Group: Voigt Lab   (2008-01-15)


DH domain constitutively active

This protein coding frame is a Guanine Exchange Factor (GEF) domain from human eukaryotic with point mutations to make it constitutively active. This protein can be secreted at high titer via the Salmonella T3S using the Voigt Lab secretion circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 88
    Illegal PstI site found at 318
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 88
    Illegal PstI site found at 318
    Illegal NotI site found at 614
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 408
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 88
    Illegal PstI site found at 318
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 88
    Illegal PstI site found at 318
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 562


[edit]
Categories
Parameters
None