Reporter

Part:BBa_J58102

Designed by: Valencia iGEM 2006   Group: iGEM2006_Valencia   (2006-10-26)

Promoter activated by OmpR-P with the reporter GFP

We use the promoter pOmpR BBa_R0082 to interface with sensors that use a synthetic two-component signal transduction pathway based on an EnvZ domain (and a EnvZ- strain such as BBa_V1012). That promoter is regulated positively (activated) by the transcription factor OmpR-P (phosphorylated).

The deep components are as follows:
OmpR
R0082

B0034
GFP
E0040

B0010

B0012
This composite part will be very useful to characterize many cellular biosensors.

By Valencia iGEM 2006

A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).

1 Universidad Politecnica de Valencia, Spain

2 Universitat de Valencia, Spain

3 Ecole Polytechnique, France


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 778


[edit]
Categories
//classic/reporter/pret
Parameters
None