Coding

Part:BBa_J52026:Design

Designed by: Jelka Pohar   Group: iGEM2006_Slovenia   (2006-10-15)


dnMyD88-linker-GFP

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

dnMyD88-linker-GFP was made by using PCR overlap extension technique. This part is cloned in BioBrick vector pSB1AK3. Original sequence has PstI restriction site so we have to introduce a mutation without affect on amino acid sequence. The part is cloned in pSB1AK3 biobrick vector.

Source

dnMyD88- GFP- more to come

References