Regulatory
p(osmY)
Part:BBa_J45992
Designed by: Stephen Payne Group: iGEM2006_MIT (2006-07-11)
Full-length stationary phase osmY promoter
BBa_J45992 is a stationary phase promoter derived from the promoter that controls transcription of osmY in Escherichia coli . BBa_J45992 is active in stationary phase and under high osmotic pressure conditions.
Usage and Biology
Expression of osmY is dependent on σS in vivo . Schellhorn et al. previously demonstrated that an osmY::lacZ fusion generated the highest transcriptional signal in stationary phase as compared to nine other σS-dependent promoter-lacZ fusions . In addition, the osmY::lacZ fusion generated only a small transcriptional signal during exponential growth.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//chassis/prokaryote/ecoli
//direction/forward
s
//promoter
//regulation/constitutive
//rnap/prokaryote/ecoli/sigmaS
//direction/forward
s
//promoter
//regulation/constitutive
//rnap/prokaryote/ecoli/sigmaS
| negative_regulators | |
| positive_regulators |

