Coding
BSMT2

Part:BBa_J45007:Design

Designed by: Boyuan Zhu   Group: iGEM2006_MIT   (2006-06-07)

SAM:benzoic acid/salicylic acid carboxyl methyltransferase II; converts salicylic acid to methyl sal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 204


Design Notes

Forward Strand (1 and 2)

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG GAA GTT GTT GAA GTT C -3'

  • LENGTH: 19
  • GC CONTENT: 36.8 %
  • MELT TEMP: 47.8 ºC

Designed Reverse Strand (phBSMT2)

5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAA GAG AC -3'

  • LENGTH: 22
  • GC CONTENT: 27.3 %
  • MELT TEMP: 47.1 ºC

Source

  • BSMT2 gene from Petunia x hybrida (GenBank AY233466)

References

<biblio>

  1. Underwood05 pmid=15849311
  2. Negre03 pmid=14630969

</biblio>